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GE28-9440-06

Protein A Mag Sepharose

Cytiva 28-9440-06, pack of 1 × 500 μL

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

native protein A

包裝

pack of 1 × 500 μL

製造商/商標名

Cytiva 28-9440-06

儲存條件

(20% Ethanol, 20% medium slurry)

參數

Room temperature temp. range

基質

paramagnetic, spherical, highly cross-linked agarose particles

容量

8- 17 mg binding capacity(human IgG/ml gel)

儲存溫度

2-8°C

一般說明

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose with native Protein A as ligand.

應用

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native Protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species.

There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.

Protein A Mag Sepharose combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

特點和優勢

  • Visible and dense Sepharose based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
  • Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


分析證明 (COA)

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文章

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

條款

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

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