推薦產品
形狀
lyophilized powder
品質等級
包裝
glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g
儲存條件
do not freeze
標籤範圍
3 mg per mL
技術
affinity chromatography: suitable
顏色
white to gray
基質
Sepharose CL-4B
基質活化
cyanogen bromide
基質結合
amino
基質墊片
1 atom
容量
≥16 mg/mL binding capacity (human IgG)
溶解度
water: 1 g/L at 20 °C
適合性
conforms to structure for swelling capacity (1g swells to 4 to 5 mL)
儲存溫度
2-8°C
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一般說明
当与抗体连接时，蛋白A-琼脂糖凝胶有助于提高交联到柱基质上的IgG的抗原结合能力。在这种情况下，结合发生在蛋白A与IgG分子的Fc部分之间，使抗原特异性位点游离。
應用
蛋白A-琼脂糖凝胶™用于亲和层析、抗体纯化和表征、免疫亲和基质、蛋白层析、蛋白A、G和L树脂以及重组蛋白表达和分析。蛋白A-琼脂糖凝胶™已用于制定研究蛋白相互作用的策略，改善乳糜泻检测，以及研究儿童糖尿病。
蛋白A-琼脂糖凝胶具有以下用途：
- 用于免疫沉淀胎盘，使用蛋白印迹技术进行蛋白质和RNA分析。
- 用于染色质免疫沉淀，以了解介体和特定启动子染色质结构重构之间的联系。
- 用作裂解缓冲液，以去除雌性大鼠脑样本蛋白质提取中的内源性G型Ig。
- 用于兔抗血清的[32P]磷肉豆蔻素C的免疫沉淀反应，以研究蛋白激酶C （PKC）和磷肉豆蔻素C （PMC）在T细胞发育过程中的下调。
數量
膨胀：1 g膨胀至约4 ml。
外觀
以用乳糖和葡聚糖稳定的冻干粉形式供应。
法律資訊
Sepharose is a trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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條款
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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