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GE28-4082-56

HiTrap® MabSelect™

Cytiva 28-4082-56, pack of 5 × 5 mL

同義詞:

HiTrap MabSelect, MabSelect column

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

包裝

pack of 5 × 5 mL

製造商／商標名

Cytiva 28-4082-56

儲存條件

(20% Ehtanol)
 (20% Ethanol)

參數

42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

highly cross-linked agarose

樣品平均尺寸

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

工作範圍

3-10

容量

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

適合性

suitable for bioprocess medium

儲存溫度

2-8°C

相關類別

一般說明

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

應用

Lab Purification using the MabSelect™ Protein A tag for capture of antibodies.

特點和優勢

  • HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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文章

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

條款

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

线性流速（cm/小时）与体积流速（mL/min)之间的换算

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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