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Key Documents

M3546

Sigma-Aldrich

超低范围分子量标记物(M.W. 1,060-26,600)

同義詞:

protein markers, protein molecular weight markers, protein standards

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About This Item

分類程式碼代碼:
41105337
NACRES:
NA.25

用途

 vial sufficient for 400 applications on large gels (16 × 18 cm)
 vial sufficient for 800 mini-gels (10 × 10 cm)

包裝

vial of 200 μL

儲存溫度

−20°C

應用

超低分子量标记物 (M.W.1,060-26,600) 已作为蛋白质标记物用于十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 和免疫印迹。

生化/生理作用

超低量程标记物设计用于 Tris-Tricine SDS-PAGE 方法 当用亮蓝 G(非甲醇基)染色时。建议使用超低标记物固定戊二醛,顺序使两种最小的蛋白质固定在凝胶中。超低量程标记物以液体形式提供,上样前必须用提供的样品缓冲液进行稀释 (1:20)。

外觀

0.1 MTris-HCl 溶液,pH 值约为 8.5,与 4 mM EDTA,3 mM 叠氮化钠和 40% 甘油

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations

條款

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)

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