推薦產品
ligand
recombinant protein A (E. coli)
包裝
pack of 200 mL
製造商／商標名
Cytiva 17-5199-02
基質
highly cross-linked agarose
樣品平均尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place
2-12
工作範圍
3-10
容量
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
適合性
suitable for bioprocess medium
儲存溫度
2-8°C
一般說明
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
MabSelect™ is a protein A affinity medium designed for capturing MAbs from large sample volumes.
MabSelect™ is a BioProcess affinity medium for capturing monoclonal antibodies (MAbs) from large sample volumes. MabSelect™ is developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect™ has been designed-in to several new and second-generation processes for the production of therapeutic MAbs.
The medium is based on a highly cross-linked agarose matrix with a recombinant protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. MabSelect™ is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.
The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect™ an excellent choice for purification of MAbs at process scale.
MabSelect™ is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, MabSelect™ meets industrial demands with security of supply and comprehensive technical and regulatory support.
MabSelect™ is a BioProcess affinity medium for capturing monoclonal antibodies (MAbs) from large sample volumes. MabSelect™ is developed for cost-effective high throughput processing of large amounts of feed. This is the reason why MabSelect™ has been designed-in to several new and second-generation processes for the production of therapeutic MAbs.
The medium is based on a highly cross-linked agarose matrix with a recombinant protein A ligand. This ligand is engineered to favor an oriented coupling resulting in an affinity medium with enhanced binding capacity for IgG. The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. MabSelect™ is optimized for high capacity, throughput and to deliver a product pool that is high in purity and yield.
The recombinant Protein A ligand is expressed in E. coli and is free of components of mammalian origin. The specificity of binding to the Fc region of IgG is similar to that of native protein A giving a high purification factor in a single step. In addition, the high capacity and the novel base matrix make MabSelect™ an excellent choice for purification of MAbs at process scale.
MabSelect™ is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, MabSelect™ meets industrial demands with security of supply and comprehensive technical and regulatory support.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
特點和優勢
- High-flow agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
- Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity which reduces media volume requirements
- Low non-specific binding resulting in low levels of impurities in the product eluate pool
- BioProcess medium supported for industrial applications and well-established in approved processes.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
MabSelect is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
分析證明 (COA)
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