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GE17-5144-01

HiTrap® Benzamidine Fast Flow

Cytiva 17-5144-01, pack of 1 × 5 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

p-aminobenzamidine

包裝

pack of 1 × 5 mL

製造商/商標名

Cytiva 17-5144-01

儲存條件

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

參數

42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

highly cross-linked 4% agarose

平均直徑

90 μm

cleaning

1-9

工作範圍

2-8

儲存溫度

2-8°C

一般說明

HiTrap® Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.

應用

HiTrap® Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.

The medium, Benzamidine Sepharose 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC System.

特點和優勢

  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
  • Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
  • HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
  • High binding capacity.
  • The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA® design or FPLC System.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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文章

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from GE Healthcare.

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

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This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

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