跳轉至內容
Merck
全部照片(1)

Key Documents

GE17-5068-01

Sigma-Aldrich

SOURCE™ 15RPC ST 4.6/100

pkg of 1 column

登入查看組織和合約定價
全部照片(1)

About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

ligand

matrix surface

材料

polypropylene column

特點

autoclavable 20 min at 121°C

包裝

pkg of 1 column

參數

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

技術

HPLC: suitable

床尺寸

4.6 mm × 100 mm

床體積

1.7 mL

柱內徑

4.6 mm

基質

polymeric
polystyrene/divinylbenzene

基質活性組

polystyrene-divinylbenzene phase

樣品平均尺寸

15 μm

cleaning

1-14

工作範圍

1-12

容量

~10 mg binding capacity

適合性

suitable for bioprocess medium

分離技術

reversed phase

一般說明

SOURCE™ 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

應用

SOURCE™ 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE™ 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE™ 15RPC offers properties superior to those of other polymeric matrices.

特點和優勢

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE™ to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

推薦產品

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

法律資訊

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

文章

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

條款

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務