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GE17-0727-20

SOURCE 15RPC

Cytiva 17-0727-20, pack of 10 mL

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About This Item

分類程式碼代碼:
13111000
NACRES:
NA.56

ligand

matrix surface

特點

autoclavable 20 min at 121°C

包裝

pack of 10 mL

製造商/商標名

Cytiva 17-0727-20

基質

polystyrene/divinylbenzene

樣品平均尺寸

15 μm (monosized)

cleaning in place

1-14

工作範圍

1-12

容量

~10 mg binding capacity

適合性

suitable for bioprocess medium

一般說明

SOURCE 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high f low velocities and low back-pressure.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

應用

SOURCE 15RPC is based on a 15 μm, monosized, rigid polystyrene/divinyl benzene matrix. This gives Low back pressures and high flow rates during operation allowing for high productivity. The 15 μm bead size allows for very high resolution chromatography steps.

High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.

A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE 15RPC is a strong alternative to silica-based RPC matrices.

SOURCE 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.

特點和優勢

  • Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
  • Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
  • High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
  • BioProcess medium supported for industrial applications.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

SOURCE is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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分析證明 (COA)

Lot/Batch Number

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文章

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

This page discusses column packing and preparation techniques for reverse phase chromatography

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

條款

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

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