GE17-0963-02

Protein A Sepharose^™ Cl-4B

Cytiva 17-0963-02, pack of 500 mL

• 分類程式碼代碼: 41106500
• NACRES: NA.56

屬性

• ligand: native protein A
• 包裝: pack of 500 mL
• 製造商／商標名: Cytiva 17-0963-02
• 儲存條件: (20% Ehtanol)
• 基質: 4% cross-linked agarose
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 2-10
• 工作範圍: 3-9
• 容量: ~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
• 儲存溫度: 2-8°C

描述

一般說明

Protein A Sepharose^™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose^™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

• Well documented for immunoglobulin purification and fractionation
• Strong binding to Fc region of IgG
• High capacity - 20 mg human IgG/mL.
• Suited for immunoprecipitation procedures

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids