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重要文件

G1544

Sigma-Aldrich

抗-GFP,N端 兔抗

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

同義詞:

抗-绿色荧光蛋白

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.56

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

濃度

~1.0 mg/mL

技術

immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates
western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

绿色荧光蛋白(GFP)生色团含具有Phe64-Ser-Tyr-Gly-Val-Gln69氨基酸序列的六肽片段。GFP结构包含11链β-桶状结构和中心螺旋,对应分子量为26 kDa。

特異性

对于GFP融合蛋白有特异性。

免疫原

合成多肽对应GFP氨基酸3-17。

應用

兔抗GFP(N末端抗体)抗体用于:
  • 蛋白质印迹
  • 组织化学和免疫组化
  • 免疫沉淀


生化/生理作用

绿色荧光蛋白(GFP)应用于光化学和诊断领域。也广泛应用于蛋白动力学研究。多克隆抗-GFP、兔抗N端抗体可与GFP融合蛋白发生特异性反应。

外觀

溶于0.01M磷酸盐缓冲盐水,pH 7.4,含有1%牛血清白蛋白以及15 mM叠氮化钠。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells
Gable T, et al.
Cancer letters, 396(6), 85-93 (2017)
Production and histological application of affinity-purified antibodies to heat-denatured green fluorescent protein
Nakamura KC, et al.
The Journal of Histochemistry and Cytochemistry, 56(7), 647-657 (2008)
Li Yang et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(1), 315-320 (2011-12-21)
Plant microRNAs (miRNAs) typically mediate RNA cleavage, but examples of miRNA-mediated translational repression have also been reported. However, the functional significance of this latter process is unknown. We identified SUO in a screen for Arabidopsis mutations that increase the accumulation
Beatriz Jiménez et al.
The Journal of biological chemistry, 287(8), 5698-5709 (2011-12-20)
Tah1 and Pih1 are novel Hsp90 interactors. Tah1 acts as a cofactor of Hsp90 to stabilize Pih1. In yeast, Hsp90, Tah1, and Pih1 were found to form a complex that is required for ribosomal RNA processing through their effect on
Green fluorescent protein (GFP): applications, structure, and related photophysical behavior
Zimmer M
Chemical Reviews, 102(3), 759-782 (2002)

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