推薦產品
共軛
magnetic beads
品質等級
形狀
(1:1 suspension in a 30% ethanol solution)
分析物化學類別
proteins (GST)
技術
immunoprecipitation (IP): suitable
protein purification: suitable
基質
4% beaded magnetic agarose
基質墊片
12 atoms
容量
≥15 μmol/mL, agarose binding capacity
運輸包裝
wet ice
儲存溫度
2-8°C
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一般說明
谷胱甘肽磁性琼脂糖珠由顺磁性的4%珠状琼脂糖组成。谷胱甘肽通过硫共价连接到环氧活化琼脂糖上，形成12个原子（10个碳）的间隔基。
谷胱甘肽磁性琼脂糖珠可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应（IP），如天然谷胱甘肽硫转移酶（GST）、谷胱甘肽过氧化物酶和乙二醛酶I，或来自细胞裂解物或其它生化溶液的GST标记重组融合蛋白，同时显示其它蛋白质的低非特异性结合。用磁铁分离结合到亲和树脂上的重组GST标记的蛋白质。磁性允许非常快速的加工，并有助于操作，例如重复洗涤和回收所需的蛋白质。这导致了更快的回收率、实验再现性和相关蛋白质的更精确定量。
磁珠基质是4%珠状琼脂糖，平均直径为50 μ；直径范围为20-75 μ。顺磁性铁浸渍在磁珠中。
谷胱甘肽磁性琼脂糖珠可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应（IP），如天然谷胱甘肽硫转移酶（GST）、谷胱甘肽过氧化物酶和乙二醛酶I，或来自细胞裂解物或其它生化溶液的GST标记重组融合蛋白，同时显示其它蛋白质的低非特异性结合。用磁铁分离结合到亲和树脂上的重组GST标记的蛋白质。磁性允许非常快速的加工，并有助于操作，例如重复洗涤和回收所需的蛋白质。这导致了更快的回收率、实验再现性和相关蛋白质的更精确定量。
磁珠基质是4%珠状琼脂糖，平均直径为50 μ；直径范围为20-75 μ。顺磁性铁浸渍在磁珠中。
應用
可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应（IP），例如来自细胞裂解物和其他生物样本的重组GST标记的融合蛋白或天然谷胱甘肽转移酶、谷胱甘肽过氧化物酶和乙二醛酶I。可用于单管纯化或96孔多孔板高通量纯化。磁珠的磁性有助于操作，如重复洗涤和蛋白质回收。这导致了更高的实验再现性和相关蛋白质的更精确定量。
外觀
30%乙醇溶液的混悬液(1:1)
訊號詞
Danger
危險聲明
危險分類
Flam. Liq. 2
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 2
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相關內容
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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