F5392

Fibroblast Growth Factor-Basic from bovine pituitary

~90% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

• 同義詞: FGF2, bFGF
• CAS號碼: 106096-93-9
• MDL號碼: MFCD00081382
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 產品名稱: Fibroblast Growth Factor-Basic from bovine pituitary, FGF-Basic, suitable for cell culture
• 生物源: bovine pituitary glands
• 品質等級: 200
• 化驗: ~90% (SDS-PAGE and N-terminal analysis)
• 形狀: lyophilized powder
• 效力: 0.03-3.0 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: 16.4 kDa
• 包裝: pkg of 1 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: <0.5 EU/vial
• 顏色: white
• UniProt登錄號: P03969
• 儲存溫度: −20°C
• 基因資訊: bovine ... FGF2(281161)

描述

一般說明

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

應用

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:

• In animal and human cell culture
• In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
• In the generation of directly reprogramming fibroblast
• In human glioma cell culture

生化／生理作用

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

外觀

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

準備報告

Purified glands by a modification of the method of Gospodarowicz, et al.

分析報告

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)