F2178

Anti-FKHRL1 (FOXO3a) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-AF6q21, Anti-FKHRL1, Anti-FKHRL1P2, Anti-FOXO2, Anti-FOXO3A
• MDL號碼: MFCD03791550
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 物種活性: mouse, human
• 包裝: antibody small pack of 25 μL
• 技術: indirect immunofluorescence: 1:200 using paraformaldyde-fixed FKHRL1 transfected 293T cells; microarray: suitable; western blot: 1:2,500 using human FKHRL1 in transfected 293T cell extracts
• UniProt登錄號: O43524
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FOXO3(2309) ; mouse ... Foxo3(56484)

描述

一般說明

Forkhead family transcription factor (FKHRL1), also known as FOX3a, is a member of the forkhead transcription factor superfamily. In FKHRL1 (FOX3a), there are three PKB phosphorylation consensus sites, Thr³², Ser²⁵³, and Ser³¹⁵. Thr³² and Ser²⁵³ are phosphorylated by PKB after induction by survival factors such as IGF-1.

免疫原

thetic peptide corresponding to the N-terminus of human FKHRL1 (amino acids 653-668 with N-terminally added cystein) conjugated to maleimide-activated KLH. This sequence is conserved in human and mouse FKHRL1.

應用

Anti-FKHRL1 (FOXO3a) antibody produced in rabbit has been used in:

• immunohistochemistry
• western blotting
• immunofluorescence staining

生化／生理作用

Forkhead transcription factor superfamily proteins act as the targets of the phosphoinositide-3-kinase–protein kinase B/Akt (PI3K-PKB) pathway. FKHRL1/FOXO3a modulates the expression of several genes that regulate DNA repair in response to stress at the G2-M checkpoint and aging. Other genes that are regulated by FKHRL1 include mitotic genes such as cyclin B and polo-like kinase (plk). Antibodies reacting specifically with FKHRL1 (FOXO3) may be useful in studying the expression and function of the protein, as well as for correlating their expression pattern with physiological functions or pathological conditions.

Forkhead1 (FOXO-3A or FKHRL-1) functions to protect cells from oxidative stress . Dephosphorylation of FOXO3a stimulates the Fas ligand gene that activates apoptosis in cells . Anti-FKHRL1 is specific for human and mouse FKHRL1. FKHRL1 immunizing peptide specifically inhibits the staining of FKHRL1.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable