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Merck
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重要文件

E7158

Sigma-Aldrich

pFLAG-CMV-4表达载体

shuttle vector for intracellular transient or stable expression of N-terminal Met-FLAG

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About This Item

分類程式碼代碼:
12352200

籤條

Met-FLAG tagged

等級

for molecular biology

形狀

buffered aqueous solution

肽標籤位置

N-terminal

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

pFLAG-CMV-4是一个6.2 kb的表达载体,用于哺乳动物细胞中的瞬时或稳定表达。该载体是一种pCMV5的衍生物,用于将正确插入的开放阅读框作为N末端Met-FLAG®融合蛋白进行瞬时或稳定表达。

pFLAG-CMV-4表达载体是一种穿梭载体,包含细菌和SV40复制起点,可在大肠杆菌和哺乳动物细胞中增殖。当使用表达SV40大T抗原的宿主细胞时,复制和基因组整合的效率最高。

FLAG表位是一个由8个氨基酸标签(DYKDDDDK)组成的小型亲水性标记物,可使用于Sigma Aldrich的ANTI-FLAG产品进行灵敏检测和高质量纯化。

pFLAG-CMV-4-BAP对照质粒是pCMV5的7.7 kb衍生物,用于N末端Met-FLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。

载体图谱和序列

成分

  • pFLAG-CMV-4 表达载体 20 μg (E1775) ,浓度为 0.5mg/ml,溶于含有 1mM EDTA 的 10mM Tris-HCl(pH8.0)中。
  • pFLAG-CMV-4-BAP 对照质粒 20 μg (C4722) ,浓度为 0.5mg/ml,溶于含有 1mM EDTA 的 10mM Tris-HCl(pH8.0)中。

原則

人巨细胞病毒2的启动子调节区可驱动FLAG®-融合构建体的转录。氨基糖苷磷酸转移酶II基因(neo-r)可表现对氨基糖苷类(如G 418)的抗性,从而可用于稳定转染子的选择。

法律資訊

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids


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