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重要文件

E5134

Sigma-Aldrich

乙二胺四乙酸 二钠盐 二水合物

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

同義詞:

Sequestrene Na₂, EDTA 二钠盐, EDTA-Na2, 乙二胺四乙酸Na2, 乙二胺四乙酸盐 二钠盐 二水合物, 二钠乙二胺四乙酸盐 二水合物, 依地酸钙钠

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About This Item

經驗公式(希爾表示法):
C10H14N2Na2O8 · 2H2O
CAS號碼:
分子量::
372.24
Beilstein:
3900609
EC號碼:
分類程式碼代碼:
12352107
PubChem物質ID:
NACRES:
NA.31

等級

for molecular biology

品質等級

化驗

99.0-101.0% (titration)

形狀

powder

反應適用性

reagent type: chelator

技術

electrophoresis: suitable

雜質

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

溶解度

3  M NaOH: 1.6 g/10 mL

正離子痕跡

Fe: ≤0.005%
Pb: ≤0.002%

適合性

suitable for electrophoresis
suitable for molecular biology

異物活動

DNase, RNase, NICKase and protease, none detected

儲存溫度

room temp

SMILES 字串

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI 密鑰

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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一般說明

脱水EDTA二钠盐可螯合金属离子,与二价阳离子形成八面体络合物。在分子生物学研究中可用于抑制依赖金属离子激活的酶。在诊断检测中,可作为采血抗凝剂用于血液学检验。

應用

EDTA二钠盐在研究中的应用:
  • 作为电泳、细胞培养、酶法检测、siRNA、流式细胞术、二甲基亚甲蓝等的缓冲液组分
  • 作为全血采集的抗凝剂
  • 作为细胞培养物样本冷冻的稳定剂
  • 与PBS和胰蛋白酶共同用于消化细胞
  • 用于污染土壤的重金属植物萃取
二价阳离子的螯合剂。 对需要二价阳离子发挥活性的酶,比如金属蛋白酶具有抑制作用。

特點和優勢

  • 分子生物学级,适于许多分子生物学应用
  • 不含内切核酸酶DNA酶、RNA酶、切口酶和蛋白酶

象形圖

Exclamation markHealth hazard

訊號詞

Warning

危險聲明

危險分類

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

標靶器官

Respiratory Tract

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Dalley AJ, et al.
Cancer Medicine, 3(2), 273-283 (2014)
External quality assessment of methylmalonic acid and total homocysteine.
M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
Erwin G Zoetendal et al.
Nature protocols, 1(2), 870-873 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that develops in time and space. The most widely used approaches to study microbial diversity and activity are all based on the analysis of nucleic acids, DNA, rRNA and mRNA.
Michi Miura et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 28(3-4), 381-393 (2020-10-19)
CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in

文章

The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.

條款

Selected Protocol from "Protein-Protein Interactions: A Molecular Cloning Manual, 2nd Edition,", Erica A. Golemis and Peter D. Adams. Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with Formaldehyde

election of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry

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