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Merck
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Key Documents

E0276

Sigma-Aldrich

EPPS

BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)

同義詞:

4-(2-羟乙基)-1-哌嗪丙磺酸, 4-羟乙基哌嗪丙磺酸, N-(2-羟乙基)哌嗪-N′-(3-丙磺酸), HEPPS

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About This Item

經驗公式(希爾表示法):
C9H20N2O4S
CAS號碼:
分子量::
252.33
Beilstein:
3957385
EC號碼:
MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:
NACRES:
NA.25

等級

BioPerformance Certified

化驗

≥99.5% (titration)

形狀

crystalline powder

技術

cell culture | mammalian: suitable

雜質

endotoxin and Total Aerobic Microbial Count, tested

pH值

7.3-8.7

有用的pH值範圍

7.3-8.7

pKa (25 °C)

8.0

pKa 

8

mp

237-239 °C (lit.)

溶解度

H2O: 25 g + 50 mL, clear, colorless

正離子痕跡

heavy metals (as Pb): ≤5 ppm

λmax

A290 at ≤0.1 (C=33% w/w)

應用

diagnostic assay manufacturing

SMILES 字串

OCCN1CCN(CCCS(O)(=O)=O)CC1

InChI

1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)

InChI 密鑰

OWXMKDGYPWMGEB-UHFFFAOYSA-N

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一般說明

EPPS or 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (HEPPS) is a zwitterionic buffer substance from the group of good buffers. It effectively maintains constant pH in a solution, which is necessary for many biological and biochemical reactions. EPPS buffer is widely used in cell culture experiments and biological research.

應用

EPPS may be used as a buffer solution to stabilize the pH in sea water samples during the titration experiments.

特點和優勢

  • BioPerformance Certified, high-quality level (400), ensures reliable and consistent results.
  • Suitable for mammalian cell culture techniques

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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存取文件庫

lonization Enthalpies of Some Common Zvvitterionic Hydrogen-lon Buffers (HEPES, PIPES, HEPPS and BES) for Biological Research
Roig T, et al.
Journal of Separation Science, 993(47), 899-90j-899-90j (1993)
Feng Xie et al.
Cancer letters, 364(2), 106-117 (2015-05-17)
Cervical cancer is often associated with eosinophil (EOS) infiltration, but the source and the role of EOS are still largely unknown. Our previous work has established that thymic stromal lymphopoietin (TSLP) can stimulate the growth of cervical cancer cell in
Xiannu Jin et al.
Journal of pharmacological and toxicological methods, 70(2), 188-194 (2014-08-26)
Malaria is a major health concern and affects over 300million people a year. Accordingly, there is an urgent need for new efficacious anti-malarial drugs. A major challenge in developing new anti-malarial drugs is to design active molecules that have preferable
Calvin R Justus et al.
Experimental cell research, 334(1), 100-113 (2015-04-08)
The effect of acidosis, a biochemical hallmark of the tumor microenvironment, on cancer progression and metastasis is complex. Both pro- and anti-tumorigenic effects of acidosis have been reported and the acidic microenvironment has been exploited for specific delivery of drugs
Anindya Sarkar et al.
The Journal of biological chemistry, 290(35), 21615-21628 (2015-07-03)
Heat shock protein 90 (hsp90) drives heme insertion into the β1 subunit of soluble guanylate cyclase (sGC) β1, which enables it to associate with a partner sGCα1 subunit and mature into a nitric oxide (NO)-responsive active form. We utilized fluorescence

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