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一般說明
REDTaq®基因组DNA聚合酶是Taq DNA聚合酶与惰性红色染料的独特混合物。这种特殊的配方旨在增强更复杂或基因组模板的扩增。REDTaq基因组DNA聚合酶高度灵敏，可提高良率并延长产品长度。它具有REDTaq DNA聚合酶的所有优点，如易于观察加酶和完全反应混合，以及直接加载到琼脂糖凝胶中。这种惰性红色染料不会影响自动或手动测序、限制消化或其他下游应用。这种染料可以用任何标准的提纯方法轻易去除。
應用
REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
特點和優勢
- 增强基因组和复杂 DNA 模板扩增
- 在实现与 Taq DNA 聚合酶相同性能的同时，具有适用于高通量应用的更方便形式
- 快速识别并确认适当混合
- 无需上样缓冲液或跟踪染料。样品可直接从反应中提取并上样至琼脂糖凝胶上
- 样品可以像嵌套PCR一样进行重复扩增
- 红色染料的迁移速度比溴酚蓝快
- 由于适当的混合，反应一致性更佳
成分
Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2
單位定義
在74°C下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。
法律資訊
本产品的使用涵盖在以下一项或多项美国专利以及美国以外的相应专利要求中：美国 8,404,464 和美国7,972,828。本产品的采购包括上述专利要求下的有限、不可转让的诉讼豁免。
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Paola Persico et al.
Veterinary dermatology, 22(6), 521-527 (2011-06-10)
Ulcerative dermatitis caused by feline herpes virus 1 (FHV-1) is an uncommon disease characterized by cutaneous ulcers secondary to epidermal, adnexal and dermal necrosis. Differential diagnoses for FHV-1 lesions include, but are not limited to, mosquito bite hypersensitivity and eosinophilic
Wiesław Bogdanowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12279-12282 (2009-07-09)
We report the results of mitochondrial and nuclear DNA analyses of skeletal remains exhumed in 2005 at Frombork Cathedral in Poland, that are thought to be those of Nicolaus Copernicus (1473-1543). The analyzed bone remains were found close to the
K L Mealey et al.
Journal of veterinary pharmacology and therapeutics, 38(5), 429-433 (2015-02-11)
The aim of this study was to sequence all exons of the ABCB1 (MDR1) gene in cats that had experienced adverse reactions to P-glycoprotein substrate drugs (phenotyped cats). Eight phenotyped cats were included in the study consisting of eight cats
條款
Method for PCR using genomic DNA or other complex templates
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