D2194

Anti-Derlin-3 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-DERL3, Anti-Der1-like domain family, member 3
• 分類程式碼代碼: 12352203
• NACRES: NA.41
• 共軛: unconjugated
• application: IF; IP; WB
• 無性繁殖: polyclonal
• 物種活性: rat (predicted), human, mouse (predicted)
• citations: 4
• 技術: immunoprecipitation (IP): 5-10 μg using extract of HEK-293T cells expressing recombinant human Derlin-3; indirect immunofluorescence: 10-20 μg/mL using human HeLa cells; western blot: 2-5 μg/mL using whole extract of HEK-293T cells expressing recombinant human Derlin-3

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~26 kDa
• 物種活性: rat (predicted), human, mouse (predicted)
• 濃度: ~1 mg/mL
• 技術: immunoprecipitation (IP): 5-10 μg using extract of HEK-293T cells expressing recombinant human Derlin-3; indirect immunofluorescence: 10-20 μg/mL using human HeLa cells; western blot: 2-5 μg/mL using whole extract of HEK-293T cells expressing recombinant human Derlin-3
• UniProt登錄號: Q96Q80
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DERL3(91319) ; mouse ... Derl3(70377) ; rat ... Derl3(690315)

描述

一般說明

Derlin-3 is a mammalian homologues of yeast Der1p, a transmembrane protein required for yeast endoplasmic reticulum (ER)-associated degradation (ERAD). Derlin 3 is encoded by the gene mapped to human chromosome 22q11.23. It is expressed in the ER and is a member of the derlin protein family.

免疫原

synthetic peptide corresponding to amino acid residues 192-205 of human Derlin-3, conjugated to KLH. The corresponding sequence differs by 3 amino acids in mouse and 2 amino acids in rat.

應用

Anti-Derlin-3 antibody produced in rabbit has been used in immunoblotting, immunoprecipitation, and immunofluorescence.

生化／生理作用

Derlin-3 is involved in the degradation of misfolded glycoproteins in the ER. Derlin-3 shares ~30% sequence identity with Derlin-1 and spans the lipid bilayer of the ER four times, showing structural similarity to Derlin-1. It is a component of the mammalian ER-associated degradation (ERAD) mechanism and is upregulated by unfolded protein response (UPR). Overexpression of this gene leads to increase in degradation of misfolded glycoprotein, whereas its knockdown blocks degradation. Derlin-3 also interacts with the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)