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D0672

Sigma-Aldrich

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

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About This Item

分類程式碼代碼:
41105335
NACRES:
NA.25

品質等級

100
200

形狀

liquid

用途

200 uses (0.1 mg size)
40 uses (20 μg size)

適合性

suitable for electrophoresis (DNA)

儲存溫度

−20°C

一般說明

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

成分

Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

準備報告

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

其他說明

For optimal resolution, the recommended agarose gel concentration is 2.0%.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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文章

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

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