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Merck
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重要文件

CS0750

Sigma-Aldrich

Caveolea/Rafts分离试剂盒

sufficient for 20 purifications

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 20 purifications

運輸包裝

dry ice

儲存溫度

−20°C

應用

该试剂盒提供了一种从组织培养细胞中获取富含小窝/脂筏蛋白的级分的简单方法。这种分离小窝和脂筏的方法是基于这些结构在冰冷的非离子洗涤剂 Triton X-100 中的不溶性。该试剂盒包括用于细胞裂解、密度梯度和分离小窝/脂筏蛋白富集部分的所有试剂,以及小窝/脂筏区室的两个标记物,抗-小窝蛋白-1和霍乱毒素 B 亚基过氧化物酶(CTB-HRP )。

生化/生理作用

小窝和脂筏是重要的富含胆固醇和鞘脂的结构,它们代表质膜的亚区室或微区,并与细胞转运和信号转导相关事件有关。

僅套裝組件

產品號碼
描述

  • Lysis buffer 100 mL

  • Cholera Toxin B Subunit, Peroxidase (CTB-HRP) 50 μg

  • Protease inhibitor cocktail for use with mammalian cell and tissue extracts 1 mL

  • Anti-Caveolin-1 antibody produced in rabbit 50 μL

  • Triton X-100 2 mL

  • OptiPrep Density Gradient Medium 100 mL

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids


分析證明 (COA)

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