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CS0340

Sigma-Aldrich

二氢叶酸还原酶检测试剂盒

1 kit sufficient for 50-100 tests

同義詞:

DHFR 检测试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

200

用途

 kit sufficient for 50-100 tests

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... DHFR(1719)

相關類別

應用

该试剂盒既可用于检测DHFR活性，也可用于筛选和DHFR抑制剂。检测原理为NADPH的DHFR能够可逆催化二氢叶酸还原为四氢叶酸，再通过监测340nm处吸光度降低情况就可以跟踪反应进程。
二氢叶酸+ NADPH+H+↔四氢叶酸+NADP+

生化／生理作用

DHFR（二氢叶酸还原酶）是一种在核苷酸碱基反应中催化生物合成DNA所必需的酶。阻断该酶就会抑制DNA合成，最终导致细胞死亡。因此DHFR也是抗肿瘤药物的出色靶点。

特點和優勢

  • 操作方法快速、简便。
  • 包含比色法测定DHFR活性所需的全部试剂，适用于细胞裂解物、组织匀浆或酶纯化柱组分。
  • 包含纯化酶，可用作阳性对照或用于筛选DHFR抑制剂。
  • 包含甲氨蝶呤（MTX），后者是一种原核和真核的DHFR特异性抑制剂，具有抗肿瘤活性。
  • 已经在A431、NIH-3T3、CHO细胞系，大鼠肝、肾、脑和骨骼肌组织提取物，以及重组DHFR中进行测试。

僅套裝組件

產品號碼
描述
  • Assay Buffer 10x for DHFR 30 mL
  • Dihydrofolate Reductase (DHFR) human .1 U
  • Dihydrofolic acid (DHFR substrate) 3 x 10
  • Amethopterin (+)(methotrexate, MTX)
    (DHFR inhibitor) 2 x 10
  • NADPH (β-Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt) 25 mg

象形圖

Skull and crossbonesHealth hazard
GHS06,GHS08

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Amrinder Singh et al.
Scientific reports, 8(1), 3190-3190 (2018-02-18)
We report the first peptide based hDHFR inhibitors designed on the basis of structural analysis of dihydrofolate reductase (DHFR). A set of peptides were rationally designed and synthesized using solid phase peptide synthesis and characterized using nuclear magnetic resonance and
Marta Jorba et al.
Antibiotics (Basel, Switzerland), 10(6) (2021-07-03)
This work reports a detailed characterization of the antimicrobial profile of two trimethoprim-like molecules (compounds 1a and 1b) identified in previous studies. Both molecules displayed remarkable antimicrobial activity, particularly when combined with sulfamethoxazole. In disk diffusion assays on Petri dishes
Bharath Srinivasan et al.
European journal of medicinal chemistry, 103, 600-614 (2015-09-29)
Gram-negative bacteria are implicated in the causation of life-threatening hospital-acquired infections. They acquire rapid resistance to multiple drugs and available antibiotics. Hence, there is the need to discover new antibacterial agents with novel scaffolds. For the first time, this study
James K Martin et al.
Cell, 181(7), 1518-1532 (2020-06-05)
The rise of antibiotic resistance and declining discovery of new antibiotics has created a global health crisis. Of particular concern, no new antibiotic classes have been approved for treating Gram-negative pathogens in decades. Here, we characterize a compound, SCH-79797, that
Prerana Ramadurgum et al.
STAR protocols, 1(2) (2020-10-01)
The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both in vitro and in vivo. Yet there are specific considerations for the development and

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