CRISPR17

CRISPR Activator Human Oct4 Positive Control Plasmid

gRNA Only Expression Plasmid

• 分類程式碼代碼: 12352200

屬性

• 品質等級: 200
• 形狀: liquid
• 包裝: vial of 50 μL
• 濃度: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• 應用: CRISPR
• 運輸包裝: dry ice
• 儲存溫度: −20°C

描述

一般說明

OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.

應用

Functional Genomics/Target Validation

• Epigenetic Modification
• Transcriptional Activation

特點和優勢

Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.

成分

1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).

聯結

To order U6-gRNA plasmids, please visit sigma.com/crisprs

Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.

其他說明

Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.

法律資訊

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable