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CPISO

Sigma-Aldrich

叶绿体分离试剂盒

isolation of intact chloroplasts from leaves

同義詞:

Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

品質等級

技術

centrifugation: suitable

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

叶绿体分离试剂盒为从植物叶片中分离完整的叶绿体提供了有效的方法。该方法包括机械式细胞壁和细胞膜破坏、过滤法细胞碎片收集和叶片组织、离心法叶绿体分离、采用Percoll®分层液或梯度法法从破碎的叶绿体之中分离完整的叶绿体。

應用

在叶绿体细胞器研究中,叶绿体分离试剂盒可用于从植物叶片中分离叶绿体以便进行结构和功能研究。

特點和優勢

  • 专门配制的缓冲液 - 采用易于执行的简单、成熟实验方案,节省时间
  • 可分离完整的叶绿体 - 适于研究碳同化、电子流和磷酸化、代谢转运或蛋白质靶向等过程
  • 总细胞器蛋白质 - 适于进行功能性研究、代谢转运、蛋白质组谱分析和基因组应用
  • 规格方便 - 节省时间,减少浪费

適合性

叶绿体分离试剂盒已采用菠菜、豌豆、生菜、卷心菜、莙达菜和烟草进行过测试。

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • Chloroplast Isolation Buffer 5x (CIB) 500 mL

  • A8022Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 gSDS

  • Chloroplast Isolation Buffer 5x (CIB) 4 ea

  • P4937Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mLSDS

儲存類別代碼

10 - Combustible liquids


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Martha Sainz et al.
Physiologia plantarum, 140(1), 46-56 (2010-05-22)
Drought and heat stress have been studied extensively in plants, but most reports involve analysis of response to only one of these stresses. Studies in which both stresses were studied in combination have less commonly been reported. We report the
Sarah Conte et al.
Plant physiology, 151(2), 559-573 (2009-08-14)
Widespread antibiotic resistance is a major public health concern, and plants represent an emerging antibiotic exposure route. Recent studies indicate that crop plants fertilized with antibiotic-laden animal manure accumulate antibiotics; however, the molecular mechanisms of antibiotic entry and subcellular partitioning
Diana V Dugas et al.
Scientific reports, 5, 16958-16958 (2015-11-26)
The Leguminosae has emerged as a model for studying angiosperm plastome evolution because of its striking diversity of structural rearrangements and sequence variation. However, most of what is known about legume plastomes comes from few genera representing a subset of
Esau Megias et al.
The protein journal, 37(3), 290-299 (2018-05-29)
Tymovirus is a genus of plant pathogenic viruses that infects several dicotyledonous plants worldwide, causing serious diseases in economically important crops. The known cytopathic effect on the host cell organelles involves chloroplast membrane deformation and the induction of vesicles in
The Complete Chloroplast Genome of 17 Individuals of Pest Species <I>Jacobaea vulgaris</I>: SNPs, Microsatellites and Barcoding Markers for Population and Phylogenetic Studies.
Doorduin, L., et al.
DNA Res., 1-13 (2011)

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