8C9

99072805, Reactivity: Anti-CD36, human

• 分類程式碼代碼: 41106514

屬性

• 生物源: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen,female
• 骨髓瘤: NS-1
• 反應: Anti-CD36, human
• 技術: cell culture | mammalian: suitable
• 同型: IgM
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse x Mouse hybridoma

細胞系描述

The hybridoma line 8C9 was produced by immunizing a female BALB/c mouse i.p. with tissue from a human malignant fibrous histiocytoma once a week for 6 weeks. Mouse spleen cells were fused with myeloma NS-1 cells using PEG and HAT for selection . The 8C9 monoclonal antibody detects a unique epitope on the leukocyte differentiation antigen CD36 (platelet glycoprotein IV or III6) expressed by normal and neoplastic endothelial cells. Immunoreactivity with 8C9 was detected on capillary endothelial cells, adipocytes, monocytes, platelets and the human monocytoid cell line U-937 (Sigma Catalogue number. 85011440). The antibody did not react with erythroid cells, T cells, B cells or HUVECs (human umbilical vein endothelial cells). Immunoblot analysis showed that 8C9 bound to a 97-kDa membrane protein expressed by U-937 cells treated with phorbol ester. Comparative immunohistochemical studies using 8C9 and the monoclonal antibody OKM demonstrated epitopic heterogeneity of the CD36 antigen by normal and neoplastic endothelial cells.

免疫原

Human malignant fibrous histiocytoma

應用

Study of endothelial cells, CD36 expression analysis

培養基

RPMI 1640 + 2mM glutamine + 10% FBS

例行更新培養

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

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