COR-L23/5010

96042338, human lung, Not specified

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: COR-L23/5010, 96042338
• 生物源: human lung
• 增長模式: Aggregates in suspension
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian lung, large cell carcinoma, drug resistant

細胞系描述

COR-L23/5010 has been derived from the parent line, COR-L23 (Sigma Catalogue number. 92031919) by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). The cells overexpress multidrug resistance-associated protein (MRP) and express a 190kDa membrane-protein connected to the degree of drug-resistance. COR-L23/5010 have a higher resistance than COR-L23/R (Sigma Catalogue number. 96042339).

應用

MRP studies, drug-resistance studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,11
D16S539: 11,13
D5S818: 10,12
D7S820: 9
THO1: 9
TPOX: 8,11
vWA: 19

培養基

RPMI 1640 + 2mM Glutamine + 1.0μg/ml doxorubicin + 10% FBS

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C It is recommended to culture the cells without drug after resuscitation until the first passage.

其他說明

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