DMS 79

95062824, human lung, Irregular

• 分類程式碼代碼: 41106514

屬性

• product name: DMS 79, 95062824
• 生物源: human lung
• 增長模式: Aggregates in suspension
• 染色體組型: Aneuploid
• 形態學: Irregular
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human lung small cell carcinoma

細胞系描述

DMS79 has been established from pleural fluid of a patient with small cell carcinoma of the lung. The patient was treated with cytoxan, vincristine, methotrexate and radiation therapy. The cultures show a tendency for the modal chromosome number to decline. The production of adrenocorticotropin (ACTH), bombesin, calcitonin, β-endorphin, 17-β-estradiol, lipotropin, ocytocin-neurophysin, parathyroid hormone and somatstatin-like imuno-reactivity has been reported. The cells have also been reported to have the following cell surface antigens: Leu-7, Class I HLA, Class II HLA, Mg23 defined by the AML-2-23 monoclonal antibody and the AML-1-99 defined antigen. The cells have a receptor for epidermal growth factor (EGF) and produce tumours in nude athymic mice. Cells grow as irregularly shaped aggregates.

應用

Human lung tumour model, carcinogenesis studies.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Maintain cultures between 3 ? 9 x 100,000 cells/ml; 5% CO₂; 37°C. Only dilute 1:2 as a maximum. This cell line grows as clumps in suspension which can be easily dispersed with pipetting. Trypsinisation is not necessary. Routine splitting can therefore

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