MCP21

96030418, Reactivity: Anti-Proteasomes, human

• 分類程式碼代碼: 41106514

屬性

• 生物源: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髓瘤: Sp2/0
• 反應: Anti-Proteasomes, human
• 技術: cell culture | mammalian: suitable
• 同型: IgG1
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse Sp2/0 x Mouse BALB/c spleen

細胞系描述

Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.

免疫原

Human placenta proteasomes

應用

Detection and purification of proteasomes and analysis of subunits

培養基

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO₂; 37°C

其他說明

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