HF1-5

94101909, rat liver, Epithelial

• 分類程式碼代碼: 41106514
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: 100 chromosomes
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 相關疾病: cancer

屬性

• 產品名稱: HF1-5, 94101909
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: 100 chromosomes
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat hepatoma Hybrid [H5xFao]

細胞系描述

HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.

應用

Gene analysis and liver function expression.

培養基

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.

其他說明

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