JEG3

92120308, human placenta, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: JEG3, 92120308
• 生物源: human placenta
• 增長模式: Adherent
• 染色體組型: modal no. 71
• 形態學: Epithelial
• 產品: Human chorionic gonadotrophin (hCG), somatomammotrophin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human choriocarcinoma

細胞系描述

One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo .

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin, 5% CO₂; 37°C. Vacuolisation will occur at confluency.

其他說明

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