3A(tPA-30-1)

96020926, human placenta, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: 3A(tPA-30-1), 96020926
• 生物源: human placenta
• 增長模式: Adherent
• 染色體組型: Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high.
• 形態學: Epithelial
• 產品: At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase.
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human placenta, SV40 transformed

細胞系描述

Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1

培養基

α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 36°C. Change medium 2-3 times weekly.

其他說明

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