推薦產品
產品名稱
HT29 gluc C1, 92012401
生物源
human colon
增長模式
Adherent
染色體組型
Not specified
形態學
Epithelial
產品
Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human Caucasian colon adenocarcinoma
細胞系描述
A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.
培養基
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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