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Key Documents

IL-A50

Reactivity: Anti-IgM (polymorphic determinant), bovine, 91072524

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About This Item

分類程式碼代碼:
41106514

生物源

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

骨髓瘤

P3X63Ag8.653

反應

Anti-IgM (polymorphic determinant), bovine

技術

cell culture | mammalian: suitable

同型

IgG2a

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

細胞系描述

The antibody is directed against a polymorphic determinant on bovine IgM. It reacts with serum IgM and IgM+ B cells, precipitating polypeptide chains of 80kDa and 28kDa. It does not react with bovine IgG.

免疫原

Bovine PBMs, depleted of T lymphocytes

應用

Evaluation of bovine immunoglobulin levels and lymphocyte populations

培養基

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation.

其他說明

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