1184

90011883, human lung, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: 1184, 90011883
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human lung fibroblast

細胞系描述

A thioguanine resistant derivative of MRC-5 CV1, selected with 5μg/ml 6-thioguanine.

應用

Genetic studies

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm³ using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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