Bu (IMR-31)

89051704, buffalo lung, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: Bu (IMR-31), 89051704
• 生物源: buffalo lung
• 增長模式: Adherent
• 染色體組型: 2n = 60
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Buffalo lung

細胞系描述

Derived from female Buffalo yearling. The cells have a finite life span of a minimum of 30 passages and are susceptible to a range of viruses including HSV, vaccinia, PRV, BVDV, para-influenza 3, bovine enterovirus (M62/4); also IBRV and VSV (Indiana). The cell line produces both detectable BVDV antigens and infectious BVDV.

應用

Virus replication studies

培養基

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

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