推薦產品
product name
ML-1, 88113007
生物源
human blood
增長模式
Suspension
染色體組型
Not specified
形態學
Lymphoblastoid
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human acute myeloblastic leukaemia
細胞系描述
ML-1 is one of three celll lines isolated in 1978 from the peripheral blood of a 24 year old male patient with acute myeloblastic leukaemia. The cells can convert to more mature cells by the use of DMSO. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
應用
ML-1 has been used to compare the cell response to mafosfamide cyclohexylamine salt, 4-hydro-peroxy-cyclophosphamide and β-D-glucose-isophosphoramide mustard, which are oxazaphosphorine agents.
DNA分析
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,12
D16S539: 9,12
D5S818: 12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,10
vWA: 16
CSF1PO: 10,11
D13S317: 9,12
D16S539: 9,12
D5S818: 12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,10
vWA: 16
培養基
例行更新培養
Maintain cultures between 2-9 x100,000 cells/ml; 5% CO2; 37°C. Freeze in 10% DMSO + 90% FBS. Immediately after resuscitation, pellet cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium. This is to remove the presence of DMSO which may cause differentiation of the cells if allowed to remain.
其他說明
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分析證明 (COA)
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