ATC-10 (Aedes aegypti)

87091801

• 分類程式碼代碼: 41106514

屬性

• 生物源: mosquito larvae
• 增長模式: Adherent aggregates
• 染色體組型: 2n = 6
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mosquito larvae

細胞系描述

Derived from pooled newly hatched Aedes aegypti larvae. The cells are susceptible to a range of arboviruses.

應用

Virus studies: Arboviruses

培養基

Mitsuhashi and Maramorosch Insect Medium (pH 7.0) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5 x10,000 cells/cm² using 0.25% trypsin for 5 mins only 28°C. After quenching the trypsin with fresh mediumdislodge the cells by gentle scraping.

其他說明

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