Ca Ski

87020501, human cervix (small bowel metastasis), Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: Ca Ski, 87020501
• 生物源: human cervix (small bowel metastasis)
• 增長模式: Adherent
• 染色體組型: Modal no. 78
• 形態學: Epithelial
• 產品: Beta Human chorionic gonadotropin (beta-hCG)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian cervical epidermoid carcinoma

細胞系描述

Derived from an epidermoid carcinoma of the cervix metastatic to the small bowel mesentery of a 40 year old Caucasian. Ca Ski cells secrete the β subunit of human chorionic gonadotropin (β-h CG), express tumour-associated antigen and G6PD type B. Ca Ski cells have been reported to contain an integrated human papilloma virus 16 genome (HPV-16 about 600 copies per cell) and HPV-18 related sequences.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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