CPAE

86111401, bovine cardiovascular , Endothelial

• 分類程式碼代碼: 41106514

屬性

• product name: CPAE, 86111401
• 生物源: bovine cardiovascular
• 增長模式: Adherent
• 染色體組型: 2n = 60, diploid
• 形態學: Endothelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Bovine pulmonary artery endothelium

細胞系描述

Established from the main stem pulmonary artery of a young, female cow (Bos taurus). The cells have angiotensin-converting enzyme activity. BVDV positive.

應用

Enzymatic studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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