RFL-6

86072501, rat lung, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: RFL-6, 86072501
• 生物源: rat lung
• 增長模式: Adherent
• 染色體組型: 2n = 42
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat Sprague-Dawley lung

細胞系描述

Derived from the lung tissue of a normal germ-free, 18 day gestation, Sprague-Dawley rat foetus. At least 45 population doublings expected.

培養基

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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