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AC2

86061902, mouse blood, Lymphoblast

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About This Item

分類程式碼代碼:
41106514
生物源:
mouse blood
增長模式:
Suspension
染色體組型:
Not specified
形態學:
Lymphoblast
產品:
Not specified
受體:
Interleukin 3 (IL-3)

產品名稱

AC2, 86061902

生物源

mouse blood

增長模式

Suspension

染色體組型

Not specified

形態學

Lymphoblast

產品

Not specified

受體

Interleukin 3 (IL-3)

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse lymphoblast

細胞系描述

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

應用

Growth factor studies, IL-2 assays

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO2; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

其他說明

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