BHK21C13-2P

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84111301, hamster kidney, Not specified

• 分類程式碼代碼: 41106514

屬性

• 生物源: hamster kidney
• 包裝: tube of 5 μg 84111301-DNA-5UG; pkg of vial of cells 84111301-1VL
• 增長模式: Suspension
• 染色體組型: 2n = 44, pseudodiploid
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Hamster Syrian kidney

細胞系描述

Derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line.

應用

Virus replication studies

培養基

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO₂; 37°C. Cells need to be grown in a techni-stirrer or they may start to re-attach.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.