MIA-Pa-Ca-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85062806, human pancreas, Epithelial

• 同義詞: MIA PaCa-2 Cells, MIA-PaCa2 Cells, MIAPACA-2 Cells, PaCa2 Cells
• 分類程式碼代碼: 41106514

屬性

• 生物源: human pancreas
• 包裝: tube of 5 μg 85062806-DNA-5UG; pkg of vial of cells 85062806-1VL
• 增長模式: Adherent
• 染色體組型: Modal no. 61; hypotriploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian pancreatic carcinoma

細胞系描述

An established cell line from an undifferentiated human pancreatic carcinoma. The tumour was taken from a 65 year old Caucasian male. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy, have a tendency to grow on top of other cells, eventually growing free in suspension. Sensitive to L-Asparaginase.

應用

MIA-Pa-Ca-2 has been used for the assessment of thyroid receptor transcriptional activity and for α-enolase immunostaining.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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