HTC (BUdR)

85061110, rat liver, Epithelial

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: HTC (BUdR), 85061110
• 生物源: rat liver
• 包裝: tube of 5 μg 85061110-DNA-5UG; pkg of vial of cells 85061110-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 66
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat liver hepatoma

細胞系描述

Clone of the original HTC hepatoma (Sigma Catalogue number. 93120108) rendered resistant to 10ug/ml BUdR, which must be grown in HAT. The cells are used in enzyme induction studies.

應用

Enzymatic studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1%HAT + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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