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Key Documents

Mero-14

9100101, human lung, Spindle shaped cells

同義詞:

Mero14 Cells

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About This Item

分類程式碼代碼:
41106514

product name

Mero-14, 09100101

生物源

human lung

增長模式

Adherent

染色體組型

Number of chromosomes 72-75

形態學

Spindle shaped cells

產品

Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

細胞系描述

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

應用

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

培養基

Hams F10 + 15% FCS + 2mM Glutamine

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs

其他說明

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分析證明 (COA)

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