Mero-25

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 9100102, human lung, Mixed epithelial and spindle shaped

• 分類程式碼代碼: 41106514

屬性

• 生物源: human lung
• 包裝: tube of 5 μg 09100102-DNA-5UG; pkg of vial of cells 09100102-1VL
• 增長模式: Adherent
• 染色體組型: Number of chromosomes 127-140
• 形態學: Mixed epithelial and spindle shaped
• 產品: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

細胞系描述

Mero-25 was derived from autopsy tumour material from a male patient aged >60 with a history of asbestos exposure

應用

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 9
THO1: 8
TPOX: 9
vWA: 16,17

培養基

Hams F10 + 15% FCS + 2mM Glutamine

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 48 hours

其他說明

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