108CC5

8062521, Mouse x Rat Hybridoma nerve, Neuronal

• 分類程式碼代碼: 41106514
• 生物源: Mouse x Rat Hybridoma nerve
• 增長模式: Adherent
• 染色體組型: Modal No. varied with passage see reference
• 形態學: Neuronal
• 產品: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• 受體: Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• 相關疾病: cancer

屬性

• 產品名稱: 108CC5, 08062521
• 生物源: Mouse x Rat Hybridoma nerve
• 增長模式: Adherent
• 染色體組型: Modal No. varied with passage see reference
• 形態學: Neuronal
• 產品: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• 受體: Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse neuroblastoma x Rat glioma hybrid

細胞系描述

The cell line108CC5 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-5 by Klee and Nirenberg . This is a cholinergic hybrid cell line.

應用

Used as a neuronal Model

培養基

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

其他說明

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