推薦產品
product name
HeLa (AC-free), 08011102
生物源
human cervix
增長模式
Aggregates in suspension
染色體組型
2n = 46, aneuploid
形態學
Spherical
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human cervical carcinoma, serum-free, animal component (AC) free
細胞系描述
HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).
應用
Virus studies
培養基
EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
例行更新培養
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
其他說明
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分析證明 (COA)
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