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Key Documents

HeLa (AC-free)

8011102, human cervix, Spherical

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About This Item

分類程式碼代碼:
41106514

product name

HeLa (AC-free), 08011102

生物源

human cervix

增長模式

Aggregates in suspension

染色體組型

2n = 46, aneuploid

形態學

Spherical

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human cervical carcinoma, serum-free, animal component (AC) free

細胞系描述

HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).

應用

Virus studies

培養基

EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

例行更新培養

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

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