RK-13 Cell Line from rabbit

rabbit kidney, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 21715, rabbit kidney, Epithelial-like

• 同義詞: GL RK/13 Cells, Glaxo Laboratories Rabbit Kidney 13 Cells, R13 Cells
• 分類程式碼代碼: 41106514
• eCl@ss: 32190102

屬性

• 生物源: rabbit kidney
• 包裝: tube of 5 μg 00021715-DNA-5UG; pkg of vial of cells 00021715-1VL
• 增長模式: Adherent
• 染色體組型: 2n=44, stem-line = 56 (=22%)
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rabbit kidney

細胞系描述

RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.

應用

Virus studies

培養基

EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.

其他說明

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