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Key Documents

C9017

Sigma-Aldrich

伴刀豆球蛋白A 来源于洋刀豆 (刀豆)

Sepharose conjugate, buffered aqueous suspension

同義詞:

ConA

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About This Item

分類程式碼代碼:
12161501
NACRES:
NA.32

生物源

Canavalia ensiformis

共軛

Sepharose conjugate

形狀

buffered aqueous suspension

包含

20% ethanol as preservative

標籤範圍

10-16 mg per mL

基質

Sepharose 4B

基質活化

cyanogen bromide

基質結合

amino

基質墊片

1 atom

容量

≥25 mg/mL binding capacity (thyroglobulin)

儲存溫度

2-8°C

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應用

用于纯化含甘露糖的糖蛋白。

生化/生理作用

刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。

外觀

悬浮于0.1 M醋酸盐缓冲液中,pH 6.0,含有1 M NaCl以及 CaCl2、MgCl2和MnCl2各1 mM

分析報告

在报道中,凝集活性以μg/ml表示,并且是在含有1mg/mL Ca 2+和Mn 2+的磷酸盐缓冲盐水(pH 6.8)的系列稀释液中进行测定的。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

102.2 °F

閃點(°C)

39 °C


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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Jae-Uoong Shim et al.
Inflammation, 32(4), 211-217 (2009-05-06)
Cudrania tricuspidata is one of the most omnipresent traditional herbal drugs for anti-inflammation and anti-tumor. The purpose of the present study was to determine whether the CTB glycoprotein regulates the inflammatory reaction stimulated by bisphenol A (BPA) in human mast
Phil-Sun Oh et al.
Journal of cellular biochemistry, 109(1), 124-131 (2009-11-04)
This study investigated the inhibitory effect of a glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) on di(2-ethylhexyl) phthalate (DEHP)-induced mast cell degranulation and related signaling cascade in RBL-2H3 cells. This experiment evaluated the intracellular Ca(2+) level, and the activities
Robert J Huber et al.
Matrix biology : journal of the International Society for Matrix Biology, 31(4), 271-280 (2012-03-07)
CyrA, an extracellular matrix (slime sheath), calmodulin (CaM)-binding protein in Dictyostelium discoideum, possesses four tandem EGF-like repeats in its C-terminus and is proteolytically cleaved during asexual development. A previous study reported the expression and localization of CyrA cleavage products CyrA-C45
Jin Lee et al.
Journal of cellular biochemistry, 112(2), 694-703 (2011-01-27)
Cadmium is one of the inflammation-related xenobiotics and has been regarded as a potent carcinogen. Gardenia jasminoides Ellis (GJE) has been used to cure inflammation in Korean folk medicine for a long time. The purpose of present study is the
Sei-Jung Lee et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 87, 12-22 (2015-12-04)
This study was carried out to investigate the anti-inflammatory potentials of a 38 kDa glycoprotein isolated from Styrax japonica Siebold et al Zuccarini (SJSZ glycoprotein). We found that SJSZ glycoprotein has concentration-dependent scavenging activity against DPPH and hydroxyl radicals in the cell-free

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