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Key Documents

C7438

Sigma-Aldrich

Chaperonin 10 from Escherichia coli

≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

同義詞:

GroES

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

Escherichia coli

品質等級

重組細胞

expressed in E. coli overproducing strain

化驗

≥95.0% (SDS-PAGE)

形狀

lyophilized powder

技術

activity assay: suitable

UniProt登錄號

儲存溫度

2-8°C

基因資訊

human ... HSPE1(3336)

生化/生理作用

Chaperonin60 (GroEL) and chaperonin10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

包裝

Package size based on protein content.

外觀

Lyophilized powder containing Tris buffer salts, potassium chloride, dithiothreitol and trehalose as stabilizer.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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